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Heat map displaying vertically infected and uninfected progeny obtained from each infected mother in vertical transmission assays conducted with non-colonized Aedes aegypti from Cuba and dengue virus type 1 <t>(DENV-1),</t> chikungunya (CHIKV), and Zika (ZIKV) viruses. Each gray box corresponds to a different mosquito progeny. Samples infected with CHIKV, ZIKV, or DENV-1 viruses are colored in blue, green, or red, respectively. M: mother, S: daughter’s saliva, H: daughter’s head, P: male pools, N/A: not assayed. Stars within the squares indicate saliva samples that tested positive by direct titration or exhibited cytopathic effect (CPE) following virus amplification in cell culture. Numbers above the colored squares representing male pools indicate the total number of males tested in each pool
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Heat map displaying vertically infected and uninfected progeny obtained from each infected mother in vertical transmission assays conducted with non-colonized Aedes aegypti from Cuba and dengue virus type 1 <t>(DENV-1),</t> chikungunya (CHIKV), and Zika (ZIKV) viruses. Each gray box corresponds to a different mosquito progeny. Samples infected with CHIKV, ZIKV, or DENV-1 viruses are colored in blue, green, or red, respectively. M: mother, S: daughter’s saliva, H: daughter’s head, P: male pools, N/A: not assayed. Stars within the squares indicate saliva samples that tested positive by direct titration or exhibited cytopathic effect (CPE) following virus amplification in cell culture. Numbers above the colored squares representing male pools indicate the total number of males tested in each pool
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Heat map displaying vertically infected and uninfected progeny obtained from each infected mother in vertical transmission assays conducted with non-colonized Aedes aegypti from Cuba and dengue virus type 1 (DENV-1), chikungunya (CHIKV), and Zika (ZIKV) viruses. Each gray box corresponds to a different mosquito progeny. Samples infected with CHIKV, ZIKV, or DENV-1 viruses are colored in blue, green, or red, respectively. M: mother, S: daughter’s saliva, H: daughter’s head, P: male pools, N/A: not assayed. Stars within the squares indicate saliva samples that tested positive by direct titration or exhibited cytopathic effect (CPE) following virus amplification in cell culture. Numbers above the colored squares representing male pools indicate the total number of males tested in each pool

Journal: BMC Biology

Article Title: Vertically infected Aedes aegypti excrete infectious arboviruses in saliva

doi: 10.1186/s12915-026-02562-2

Figure Lengend Snippet: Heat map displaying vertically infected and uninfected progeny obtained from each infected mother in vertical transmission assays conducted with non-colonized Aedes aegypti from Cuba and dengue virus type 1 (DENV-1), chikungunya (CHIKV), and Zika (ZIKV) viruses. Each gray box corresponds to a different mosquito progeny. Samples infected with CHIKV, ZIKV, or DENV-1 viruses are colored in blue, green, or red, respectively. M: mother, S: daughter’s saliva, H: daughter’s head, P: male pools, N/A: not assayed. Stars within the squares indicate saliva samples that tested positive by direct titration or exhibited cytopathic effect (CPE) following virus amplification in cell culture. Numbers above the colored squares representing male pools indicate the total number of males tested in each pool

Article Snippet: Virus stocks were generated using a multiplicity of infection of 0.1, after passages on Vero cells (ATCC, ref. CCL-81) for CHIKV and ZIKV, and C6/36 cells (ATCC, ref. CRL-1660) for DENV-1.

Techniques: Infection, Transmission Assay, Virus, Titration, Amplification, Cell Culture